Products > Life Therapeutics > von Willebrand Assay

von Willebrand Disease von Willebrand Factor (vWF) is an important blood clotting protein involved in both assisting platelet adhesion and stabilization of clotting factor VIII. In von Willebrand Disease (vWD), there is either a partial quantitative deficiency or shortage of vWF (classified as vWD Type 1) or a qualitative or functional deficiencey (classified as vWD Type 2). vWD Type 3 is rare and charachterized by virtually complete deficiency of vWF. The incidence of vWD worldwide is estimated at 1% to 3% but may be more common since mild cases may remain undetected. Historically, a wide variety of assays have been used to characterize vWF. Currently the most common methods are vWF:AG assay by Laurell rocket electrophoreses, ELISA or Latex Immunoassay and Ristocetin Cofactor agglutination (RiCoF) or Ristocetin induced platelet aggregation (RIPA). These two methods in combination can identify vWD types 1 and 2A which represent some 85% of vWD cases. Recently, the Collagen Binding Assay (CBA) has been mentioned as a useful alternative to the Ristocetin Cofactor assay for detecting variants of vWF with defective adhesive function. This is an ELISA-like method based on the ability of vWF to bind collagen, which is associated with higher molecular weight multimers of vWF. The CBA assay correlates closely with vWF function and bleeding problems.

von Willebrand Factor Antigen / Collagen Binding Assay

Life Therapeutics have developed a paired ELISA system for the quantitation of von Willebrand Factor antigen (vWF ELISA) and the binding of von Willebrand Factor to collagen type 3 (CBA ELISA). von Willebrand Factor ELISA assay kit The vWF ELISA kit is based on vWF in patient plasma binding to antibody coated wells; an antibody conjugate is then introduced to allow chromogenic quantitation of bound vWF. Collagen Binding Assay Elisa assay kit The Collagen-Binding Assay was developed to detect variants of vWF with defective adhesive function, based on the ability of vWF in patient plasma to bind to collagen coated on the ELISA plate. This assay provides enhanced discrimination between Type 2 vWD and normal patients, as well as a greater predictive power in confirming vWD Type 2 compared to traditional platelet aggregation assays. Assay variability is marketdly reduced. VWF/CBA ELISAs are Simple to use Fast - 2hrs 40 minute total Reproducable Less complex and more precise than platelet aggregation methods using ristocetin. Less time consuming than electrophoresis and aggregation methods. Product Code VWAE-1 von Willebrand Antigen ELISA kit    1 x 96 well Elisa kit CBAE-1 Collagen Binding Assay ELISA kit   1 x 96 well Elisa kit

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